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Separation of MUA During ON and OFF Periods: For 20 Hz (40 Hz) stimulation, despite using a 10 ms pulse (5 ms), corresponding to a 20% duty cycle, a neural rebound was observed immediately after stimulus offset
Supernatants were assayed for total protein concentration with Bradford assay (BioRad 5000006) and 50100 g of protein was mixed with loading buffer and loaded in a 12 or 16% gradient gel (Thermo 1Fisher XP00162)
Not to mention, some of these individuals saw some weight loss
Al-Rooqi, M
Hippocampal slices from P4 Sprague-Dawley rats were also cultured and biolistically transfected with tomato fluorescent protein to visualize dendritic arbors
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