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In standard 7H9 media vadK , vadK:vadK His422Ala , vadK:vadK His422Asp and vadK:vadK Arg106Lys Mtb strains grew slower than the parental and complemented strains, but after 21 days incubation, there was no significant differences between the strains (Appendix Fig

Some protocols add 0.6% acetic acid for better solubility