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When the body experiences stress, it releases hormones that can heighten bladder sensitivity and increase pain perception

Microbes were incubated overnight in rich liquid medium (2% proteose peptone (Thermo Fisher Cat#LP0085), 0.5% NaCl (Mercury Cat#1064041) and 0.5% yeast extract (Merck Cas#8013-01-2)) supplemented with 250 mg/mL glucose (Mercury Cas#50-99-7), 250 mg/mL K 2 HPO 4 (Holland Moran Cas#7758-11-4), 50 mg/mL L-cysteine (Merck Cas#52-90-4), 5 mg/mL Hemin (Mercury Cat#3741) and 5 L/mL vitamin K 1 (Merck Cas#84-80-0) under anaerobic conditions