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Nine epididymal white adipose tissues (3 tissues/group) were ground by liquid nitrogen into cell powder, lysed, and extracted in SDT (4%SDS, 100mM Tris-HCl, 1mM DTT, pH7.6) buffer

p-eIF4E staining was done using mouse anti-elF4E (pS209) (BD 560229) diluted 1:100 and cells were fixed with BD Cytofix/Perm and intracellular staining was done in BD Perm (BD 554714)