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No established loading or maintenance schedule Fixed protocols such as weekly loading doses followed by biweekly or monthly maintenance are commercial practice patternsnot evidence-based universal schedules proven to improve long-term health

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A multicellular tumor spheroid is a tissue culture model that mimics the pathological conditions of solid tumors including a three-dimensional cellular structure, nutrition, and proliferation gradients

doi:10.1016/s0024-3205(00)00803-1