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Each sample was thereafter incubated with 10 l of acetonitrile and stored at 20 C before analysis was performed on a Beckman HPLC instrument operated with System Gold coupled to diode array UVVIS detector module 168

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Alanine mutations confirmed that Trp221 and His223 at the surface groove are critical in the formation of the - glucan binding site on Dectin-1, and this site finds to be conserved among Dectin-1 of murine, chimpanzee, rhesus monkey, cow, and humans (168)

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