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Western blotting Myocardial tissue was lysed in radioimmunoprecipitation assay buffer (RIPA) lysate (AR0102-30, BOSTER, 30 ml) and ethylenediaminetetraacetic acid (EDTA)-containing protease inhibitor (100 ) buffer before extraction of the protein and was sonicated to fragment the myocardial tissue
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Peripheral neuropathy, subacute combined degeneration of the spinal cord, or cognitive impairment indicate more advanced deficiency and generally require intensive treatment over extended periods
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