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In order to ensure that it doesn't denature, it's best to be kept airtight before use, which is why it in produced in oral syringes
After treatment, the cells were washed with cold PBS and lysed in RIPA buffer (Biosesang, Republic of Korea) supplemented with a protease inhibitor cocktail (Merck, Darmstadt, Germany)
TB-500 acts on actin filament modulation, improving cellular migration, wound healing, and overall tissue recovery
This is one of the most effective synergies in the entire protocol
It means the 28-day discard rule is not driven by preservative exhaustion
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