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Consistency: Missing doses, inconsistent injection timing, or halfhearted diet adherence dramatically reduces effectiveness
Measurable body composition changes usually become apparent by weeks 2-4, with progressive improvements continuing through weeks 4-12 of consistent use
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In this assay, the 30 min incubation duration was chosen to be optimal because of the inherently cytotoxic nature of CuCl 2 and Cu(OAc) 2 towards HaCaT keratinocytes, as they were observed to cause substantial cell death at a duration longer than 30 mins
