Figure 6 Mitochondrial Functional Markers There was no significant difference in MnSOD, Tom-20 and OXPHOS complexes among the three groups at P1 (Figures 7A,D,G)
Cannabinoids protect cells from oxidative cell death: a receptor-independent mechanism
Materials and Methods Chemicals Ferric chloride, di-sodium hydrogen phosphate (Na 2 HPO 4 ), hydrogen peroxide (H 2 O 2 ), di-potassium hydrogen phosphate (K 2 HPO 4 ), potassium di-hydrogen phosphate (KH 2 PO 4 ), and carboxymethylcellulose (CMC) were procured from MERC, India Ltd., Mumbai, whereas hesperidin, 1-Chloro-2,4-Dinitrobenzene (CDNB), phenazine methosulphate, nitroblue-tetrazolium, sodium pyruvate, cumene hydroperoxide, thiobarbituric acid, 5-5-dithiobis[2- nitrobenzoic acid, 5-thionitrobenzoic acid, mM tert-butylhydroperoxide and nicotinamide adenine dinucleotide (NADH) were supplied by the Sigma Aldrich Chemical Company, Kolkata, India Animal care and handling The animal care and handling were performed in accordance with the guidelines issued by the World Health Organization, Geneva, Switzerland and the INSA (Indian National Science Academy, New Delhi, India)
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13,14,15 Additionally, impaired neurotrophic support and autoimmune mechanisms may further exacerbate neuronal degeneration