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The kit uses an improved 5,5'-dithiobis(2-nitrobenzoic acid) (DTNB) method that combines deproteination and detection into a single reagent, with DTNB reacting with reduced glutathione to form a yellow product

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First, publication bias and small-study effects cannot be excluded, and many trials do not stratify by obesity, phenotype, or baseline 25(OH)D, key modifiers of response

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