This analysis was additionally done in N2a cells co-transfected with GFP-fusions, HA-tagged zDHHCs or empty pcDNA3.1(+), and MannII-BFP as the Golgi marker (2:4:1 plasmid ratio)
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Histological and histomorphometric analysis The grafted femurs were harvested, fixed in 10% neutral buffered formalin (Sigma-Aldrich), decalcified in 10% EDTA (Sigma-Aldrich)
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6 ALC has been used as an adjunct treatment for bipolar depression (BD), however no significant benefits noted in improvement in depressive symptoms