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The mitotic cell cycle consists of four phases: the postmitotic interphase or Gap1 (G1), known to be influenced by environmental cues, the DNA synthesis (S) phase, the post-synthetic interphase or Gap2 (G2), and the mitotic (M) phase (Komaki and Sugimoto 2012)
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Some researchers use a designated clean space or even a small laminar flow hood for reconstitution
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