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The resin was washed with three column volumes (CVs) of PBS buffer containing 20 mM imidazole, and bound proteins were eluted with 7 mL PBS buffer containing 250 mM imidazole

Jay Gopalakrishnan PhD Heinrich-Heine-Universitt | Universittsklinikum Dsseldorf "The assay is a homophilic interaction mediated cell adhesion on purified protein (in this case, immobilized purified Pcdhga9 to Pcdhga9 expressed on cell surface)

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