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In Vitro Depletion of GSH and Generation of ROS by TNBS To examine the interaction of GSH with TNBS and the subsequent effect on GSH regulation, to an aqueous solution of GSH (1 mm) in Krebs-Henseleit buffer, an equimolar amount of TNBS was added and the level of GSH was examined by HPLC as described previously, which monitors reduced and oxidized GSH (Fariss and Reed, 1987)
Glutathione is a substance that the body can make itself from the amino acids cysteine, glycine and glutamic acid
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These methods combined allow for the simultaneous identification and imaging of various free radicals, providing metabolic and biochemical data revealing significant differences between whole-cell and mitochondrial metabolite profiles (Krishnamurthy et al., 2024)
Vitamin C and immune function
