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Any persistent or concerning changes in sexual function warrant proper medical evaluation to identify underlying causes

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Then the brains and serum were collected (see ESM Methods: Immunohistochemistry) for analysis of secondary outcomes (stroke volume, number of neurons positive for the neuronal nuclear marker [NeuN] and serum IGF-1 levels, see ESM Methods: Ischaemic stroke volume assessment and ESM Methods: ELISA immunoassays)