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The observed effects were linked to the suppression of mitochondrial disruption and diminished levels of cleaved caspase-3, achieved through a reduction in the production of mitochondrial reactive oxygen species (ROS)
The membrane was then incubated at room temperature in blocking solution for 1 hour, followed by incubation in blocking solution containing the appropriate dilution of either phospho-Jak2 or the primary antibody for growth hormone receptor, for 2 hours
& Mitra, S
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