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Cells were then lysed in ice-cold cytoplasm extraction reagent I (CER I) for 10 min and ice-cold CER II for 1 min

Secondary outcomes Five included studies comprising 3605 patients reported change in MCV, four studies comprising 140 patients reported change in homocysteine levels, four studies comprising 3588 patients reported change in platelets count, and only three studies with 3430 patients reported change in WBC count

So that's a recycle process

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Expert Treatment A quick injection is administered into the upper arm or thigh