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PLoS ONE 7 , e51040 (2012)
The primary antibodies included anti-p53 (1:200, mouse, Abcam, ab131442), anti-p53 (1:200, rabbit, Proteintech, 10442-1-AP), anti-AKT1 (1:200, rabbit, CST, 2938), and anti-progerin (1:25, mouse, Santa Cruz, sc-81611)
Metabolism : Valproate undergoes hepatic metabolism
Cells were subsequently washed with PBS, resuspended in FACS buffer and transferred through a 40m cell strainer for flow cytometry
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