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Membranes were incubated overnight at 4 C with a range of 1:10002000 dilutions of AMPK, pAMPK, ACC, pACC, TRPA1, IL-1, TNF-, cyc, and -actin primary antibodies, followed by HRP-conjugated secondary antibodies

This effect has been linked to ER UPR-signaling (118, 119, 141), and ER stress has also been demonstrated to counteract epithelial stemness (142)

Tirzepatide also employs a gradual dose escalation, starting at a subcutaneous dose 2.5 mg/week and increasing up to 15 mg/week, with no routine age-based dose adjustment required [36, 39, 40]

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