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In brief, HUVECs were lysed in Co-IP buffer containing 20 mM Tris-HCl (pH 7.5), 100 mM NaCl, 0.5 mM EDTA, 0.5% NP-40, 0.5 mM PMSF, and 0.5% protease inhibitor cocktail on ice for 20 min
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The combination produces additive hypoglycemia and has been associated with hospitalizations in research-community reporting
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