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Prepared solution: Stability depends on concentration, pH, diluent, sterility, container, preservatives, and temperature

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For example, the multienzyme complex pyruvate dehydrogenase [7] at the junction of glycolysis and the citric acid cycle requires five organic cofactors and one metal ion: loosely bound thiamine pyrophosphate (TPP), covalently bound lipoamide and flavin adenine dinucleotide (FAD), cosubstrates nicotinamide adenine dinucleotide (NAD + ) and coenzyme A (CoA), and a metal ion (Mg 2+ )

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-KG is an essential cofactor for -KG-dependent dioxygenases and is required for DNA and histone demethylation (amino acids involved in epigenetic modification will be addressed in section Amino Acid Metabolism and Epigenetics)