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Immunoprecipitation was then performed using the Catch and Release Immunoprecipitation Kit (EMD Millipore), according to the manufacturers instructions with 250 g of total protein from cells or 400 g from tissues, and 1 g of an anti-IGF-1R antibody (#9750, Cell Signaling)
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The mechanism underlying colistin-induced nephrotoxicity is not fully understood, but it arguably initiates with extensive active reabsorption and accumulation of the drug in proximal tubular cells
A conservative estimate suggests that a 50-pound weight regain costs $5,000 to $15,000 in direct healthcare costs over the following five years through increased medications, more frequent provider visits, and management of weight-related complications
Simple, convenient option for consistent antioxidant support
