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The probe plate was then prepared with mitochondrial inhibitors: 25 l 5 M rotenone (Sigma-Aldrich, 45656) and 5 M antimycin A (Sigma-Aldrich, A0149)

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The analysis was performed using gradient solvents (A and B) containing 10 mM ammonium formate at a flow rate of 0.2 mL/min