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2022;379:e070499

DSIP has been investigated for its role in sleep regulation, neuroendocrine modulation, and stress response across numerous preclinical studies over more than four decades

Isolation and culture of primary proximal tubule epithelial cells (PTECs) Fresh mouse renal cortex was minced on ice and digested in HBSS with 0.75 mg/mL trypsin inhibitor and 0.75 mg/mL collagenase at 37 C for 20 min

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