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To verify that the SAT1 disruption cassettes were successfully excised from GDH2/gdh2 and GDH3/gdh3 strains, genomic PCR was used with primers C1 and C4 to amplify the region between the upper end of 5 flank sequences to the lower end of 3 flank sequences to make sure that both alleles of each gene were removed (Supplementary Fig
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Clinical photo of the buccal mucosa of a middle-aged, black woman with a brown-black, irregularly bordered macule that arose suddenly
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