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For affinity fusion proteins, the appended protein will bind to a third, typically small, molecule that is immobilized on the surface of a polymer resin

Funding This work was supported by the Key Research and Development Program of Xinjiang Uygur Autonomous Region of China [2022B03019-1-1] and Youth Project of the Institute of Medical Sciences, Xinjiang Medical University [YXYJ20230301]

Both require careful concentration planning to keep draw volumes within a single syringe pull