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Darkening of the solution may occur over time

The American Association for the Study of Liver Diseases (AASLD) guidelines note that dietary copper restriction (below 1 mg/day) is recommended during the first year of Wilson disease treatment [2]

Without adequate bioavailable copper, newly formed collagen fibers remain structurally weak and prone to degradation

Areas of investigation include: nitric oxide signaling pathways endothelial cell migration and angiogenesis fibroblast proliferation extracellular matrix remodeling inflammatory response modulation These mechanisms are commonly studied in experimental models involving connective tissue recovery and vascular repair responses

The overlapping yet distinct mechanisms that may support coordinated repair biology, though combination evidence remains early and primarily exploratory