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ACS Omega 5 , 1258312595 (2020)

Hippocampal slices from P4 Sprague-Dawley rats were also cultured and biolistically transfected with tomato fluorescent protein to visualize dendritic arbors

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In contrast, there are studies revealing that the activation of HIF1 signaling is not principally regulated by mutant IDH1, but more likely via other recognized mechanisms like hypoxia, transcriptional regulation, and major genetic and epigenetic alterations induced by gain-of-function or loss-of-function mutations 30

While this effect might be subtle, in high-precision research, consistency is everything