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Successful isolation of monocytes was confirmed with flow cytometry (BD Fortessa) using VivaFix Viability Assay (BioRad), a cocktail of antibodies from BioLegend: anti-CD3 PE (300456), anti-CD14 BV650 (301835), anti-CD19 PerCP-Cy5.5 (302229), and anti-CD56 APC (318309)
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For these populations, B12 injections move from optional to essential
Good fats from avocados and nuts are an important part of a balanced diet
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