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After 12 h incubation, cells were treated with PBS, Ru(II)-OH, NP2, or NP4 for 24 h (Ru 10 M), washed with PBS and incubated with Annexin/PI reagent in the dark for 15 min at 25 C

However, the FDA has not approved it for human use

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Lastly, epithalon plays a significant role in the endocrine system by increasing levels of endogenous hormones that naturally diminish with age, such as melatonin (which helps regulate the sleep cycle), and gonadotropic hormones (FSG, LG, prolactin), which play a role in reproductive functions [8]

VDR regulates mitochondrial function as a protective mechanism against renal tubular cell injury in diabetic rats