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The pre-dissolved solution is by nature less stable than the lyophilisate, and after the first opening of the applicator the stability window shortens
So to put it crassly, is your method just for the rich
If freezing is necessary to extend shelf life, aliquot the solution into single-use fractions first so each small vial is only thawed once
Many of the available competitive-binding assays methods have been automated (Chan, 1996)
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