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Consequently, lower citrate ( m + 2)/PA ( m + 16) and malate ( m + 2)/PA ( m + 16) ratios were detected in shGDH1 cells (Supplementary Fig

Isolation of WAT stromal vascular cells and adipocyte differentiation To isolate the stromal vascular fraction, minced iWAT (combined depots from 45 mice per genotype) from 46-week-old mice was digested in 1 mg ml 1 collagenase D (Roche) with 1.5% BSA in sterile Hanks Balanced Salt Solution and incubated in a 37 C shaking water bath for 1 h

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