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The American Journal of Clinical Nutrition, 98 (2), 355356
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Membranes were blocked in 5% non-fat milk in Tris-buffered saline with 0.1% Tween-20 (TBST) for 1 h at room temperature and incubated overnight with primary antibodies at dilutions specified in Supplementary Table 26
This condition occurs when the arteries in the lungs become narrowed and stiff, forcing the right side of the heart to work harder
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