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At an OD 600 of 0.60.7, expression was induced with 1 mM IPTG and the culture was continued up to an OD 600 of 3.0 where the cells were harvested at the end of the exponential growth phase by centrifugation (10 min, 3,220 g , 4C)

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Fletcher MM, Keov P, Truong TT, Mennen G, Hick CA, Zhao P, et al

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Fortified tortillas made from masa produced through the traditional nixtamalization and wet-milling process, where folic acid was added to nixtamal before milling, contained 15%, 33%, and, in one study, even 80% less folic acid compared to the folic acid amount added to nixtamal (theoretical fortified level)