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Blood samples were collected at 0, 0.25, 0.5, 1, 2, 4, 6, and 15 hours after alcohol consumption to measure both alcohol and acetaldehyde levels using validated assay kits, capturing key pharmacokinetic parameters such as maximum serum concentration (Cmax) and area under the curve (AUC)
Interspecies scaling (for example, body-surface-area conversion) typically reduces a rodent mg/kg figure severalfold, and even a properly scaled number would still be a hypothetical exposure , not a validated dose
However, accumulating ChIP-seq data indicates that NRF2 predominantly binds intronic or distal intergenic regions (Malhotra et al, 2010
