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Check your serum's pH, and if it's outside 4.55.5, adjust with citric acid or sodium hydroxide
For cFos studies, two to six sections per mouse at the level of the hypothalamus (bregma 0.46 to 1.06 mm) and hindbrain (bregma 7.2 to 7.76 mm) were counted bilaterally and averaged across sections for each mouse
Acta Biomembr
References Cooper GJS, Willis AC, Clark A, Turner RC, Sim RB, Reid KBM
"We simply do not have the human data to make confident recommendations about BPC-157 for GI healing," noted a 2022 editorial in Peptides reviewing the state of the field
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