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Discarding the upper plasma, wash with three times volume of PBS for 3 times (re-suspend blood cell with PBS, centrifuge at 600 g for 10 minutes), add equal volume of Reagent I

Front Pharmacol 9:785

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However, toxins such as environmental pollutants, drugs, and chemicals in food can impair the methylation pathway by interfering with the enzymes involved in the process and depleting the body's stores of crucial components like SAMe and methionine

This targeted mechanism is central to its role in metabolic research