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The soluble fraction (lysate) was separated from the cell debris by centrifugation at 20,000 g for 20 min at 4C
Cryo-electron microscopy studies (Li et al., 2024, Cell Discovery) revealed the peptide adopts a single continuous helix achieving balanced activation through conserved interactions and receptor-specific transmembrane domain contacts
Remove it and set it aside you will reuse it to seal the spray between doses
O., Ziegler, M
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