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Thermo Fisher Scientific, Waltham, MA, United States) was added, mixed, vortexed, and incubated at 4 C overnight

This signalling divergence IGF-1R-independent ERK activation vs IGF-1R-dependent Akt/mTOR activation is one of the central research questions in the MGF field and is the mechanistic basis for the functionally distinct roles of MGF (proliferation, satellite cell activation, anti-differentiation) and mature IGF-1 (differentiation, protein synthesis, hypertrophy) in the sequential muscle regeneration programme

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They are applied directly onto the skin