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2.9 Generation of reactive oxygen species Intracellular reactive oxygen species (ROS) production was assessed using the 2,7-dichlorodihydrofluorescein diacetate (DCFH-DA) assay ( g for 5 min at 4 C, washed to remove excess DCFH-DA, and resuspended in PBS to a final hematocrit of 10%

Mutat Res Genet Toxicol Environ Mutagen 586:97101

Alternate the injection sites, especially with subcutaneous injections, to avoid irritation

*Correspondence: Yongping Liu, [email protected] These authors have contributed equally to this work and share first authorship This article was submitted to Autoimmune and Autoinflammatory Disorders : Autoimmune Disorders, a section of the journal Frontiers in Immunology Disclaimer All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers

2%) were infrequent [11]