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Formulation F1, containing Tween 80, produces smaller vesicles due to increased hydrophobicity and decreased surface energy, resulting in smaller particles compared to other formulas 55

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The purified non-acylated tirzepatideGLP-1Rmini-G s Nb35 complex (3.5 L) was applied to glow-discharged holey carbon grids (Quantifoil R1.2/1.3, 300 mesh), and subsequently vitrified using a Vitrobot Mark IV (ThermoFisher Scientific) set at 100% humidity and 4C

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