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Hyper-activated motility in sperm capacitation is mediated by phospholipase D-dependent actin polymerization

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Total internal reflection fluorescence microscopy imaging of granule mobility Cells were imaged using total internal reflection fluorescence (TIRF) microscopy (AxioObserver Z1 with a 100/1.45 objective [Carl Zeiss] and a diode-pumped solid state laser at 491 nm)

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