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2.8 ELISA tests for SIRT1, AMPK, p-AMPK, and Nrf2 levels Levels of SIRT1 (Cat# SL1254Ra), AMPK (Cat# SL1695Ra), p-AMPK (Cat# SL0570Ra), and Nrf2 (Cat# SL0985Ra) in kidney tissues were assessed using the ELISA method, following the manufacturers protocol (SunLong Biotech Co., Ltd., Hangzhou City, Zhejiang, China)

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