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Furthermore, distinguishing genuine neuroprotective effects from symptomatic improvement requires the use of robust, disease-relevant biomarkers and long-term longitudinal studies

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Further, MTX increased the expression of PERK (Figure 6B), GRP78 (Figure 6C), ATF4 (Figure 6D), and CHOP-10 (Figure 6E) in the liver compared to CTRL, indicating that ER stress is implicated in MTX-induced hepatotoxicity

To evaluate whole intestinal permeability, non-degradable probes such as sucralose or erythritol, which remain unaffected by bacteria in the colon, are added to classical DST, resulting in the so-called triple sugar test