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Months 7 through 12: long-term adaptation The second half of the first year is generally smooth from a side effect perspective
On its own, REMD2.59 had a small stimulatory effect on glucagon secretion at 1 mmol/l glucose alone (~30%)
Cell transfection The shVRK2-expressing and VRK2-overexpressing plasmids were purchased from Genechem (Shanghai, China) and then used for constitutive expression of shRNA or cDNAs
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