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doi: 10.1016/s1470-2045(13)70076-7 2 ShahCViciniFA
The assay method is based on monitoring the generation of GSH from glutathione disulfide (GSSG) by the action of glutathione reductase in the presence of reduced nicotinamide adenine dinucleotide phosphate (NADPH)
Consistently, a complementary strategy showed that in aged mice, where IGF-I levels are decreased, cardiac overexpression of IGF-I significantly improved cardiomyocyte contractile function[283], attenuating oxidative stress-mediated protein damage, normalizing Ca 2+ homeostasis, reversing age-related alterations in the expression of pro-apoptotic proteins, and decreasing the apoptosis rate[284]
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