Degradation assay Cells were treated in serum-free medium plus 11 mM glucose agonist (100 nM) before lysis (20 mM Tris base, 150 mM NaCl, 1 mM EDTA, 1 mM EGTA, 0.5% Triton X-100 plus complete mini EDTA-free protease inhibitor (Roche)), addition of urea sample buffer (100 mM Tris-HCl pH 6.8, 2.5% SDS, 4 M urea, 100 mM DTT, 0.05% bromophenol blue), 10 min incubation at 37 C and immunoblotting
C., Cavalcante, D
This is something Dr
Ilic, S., Drmic, D., Franjic, S., Kolenc, D., Coric, M., Brcic, L., Klicek, R., Radic, B., Sever, M., Djuzel, V., Filipovic, M., Djakovic, Z., Stambolija, V., Blagaic, A
Preclinical and clinical studies demonstrated its ability to activate hypothalamic neurons and increase sexual arousal