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Notably, SLC38A9 is necessary for not only leucine but also other essential amino acids (e.g., glutamine, tyrosine, and phenylalanine) that are generated via lysosomal proteolysis and efflux from lysosomes in an arginine-stimulated fashion to activate mTORC1 (Rebsamen et al., 2015

We reason that, aside from the slight difference (one amino acid change) in the sVLP sequence, several unique traits of our expression strategy may have contributed to the high-level production of PV1 D-antigen

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