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21 DTNB Colorimetric Method Principle: DTNB reacts with the sulfhydryl group of glutathione to produce yellow-colored 5-thio-2-nitrobenzoic acid, which has a maximum absorption peak at a wavelength of 412 nm, while DTNB has almost no absorption peak above 400 nm

Morgenstern AS, Greenspan L, Urbanski C